short communication: the study of igg immunoglobolin anti cryptosporidum parvum in infected neonatal balb/c mice

objective: cryptosporidiosis is a parasitic disease that is causing small protozoan genus cryptosporidum and transmission take place through fecal- oral by direct contact or indirectly through food or drink. the aim of this study was detection of anti-cryptosporidum parvum immunoglobulin igg, in newborn balb/c infected with c. parvum. materials and methods: oocysts of c. parvum were obtained from the feces of diarrheic lambs and following purification they were suspended in 2.5% aqueous potassium dichromate solution and stored at 4°c. forty suckling balb/c (3–4 days old) were divided in 8 groups (4 case groups and 4 control groups) each group consist of 5 suckling balb/c. the mice in case groups were infected oraly with 105 c. parvum oocysts, and the mice in control groups served as non-infected. blood samples were collected at 6, 9, 12 and 16 days post-infection (pi). immunoglobulin igg were extracted by salting out method and confirmed with sds-page. results: antibodies were analyzed by western blot and increased secretion of igg was confirmed in neonatal mice infected with cryptosporidum oocysts. mean od of immunoglobulin igg increased from 0.350 ± 0.099 to 0.6776 ± 0.099 in case groups but in control groups the increase was from 0.244 ± 0.016 to 0.322 ± 0.16 (p